Journal: Microbiology Spectrum

Article Title: Reference-Grade Genome and Large Linear Plasmid of Streptomyces rimosus : Pushing the Limits of Nanopore Sequencing

doi: 10.1128/spectrum.02434-21

Figure Lengend Snippet: Plasmid DNA of S. rimosus ATCC 10970, M4018, and R6-500 separated by pulsed-field gel electrophoresis (PFGE). Determination of plasmid size in the S . rimosus strains ATCC 10970, M4018, and R6-500. M, lambda PFG ladder marker (New England BioLabs).

Article Snippet: In the ATCC 10970 chromosome, this region was found to exist in two copies, first at 4680190 to 4686883 and the second at 6119904 to 6113211 bp.

Techniques: Plasmid Preparation, Pulsed-Field Gel, Electrophoresis, Marker

Journal: Microbiology Spectrum

Article Title: Reference-Grade Genome and Large Linear Plasmid of Streptomyces rimosus : Pushing the Limits of Nanopore Sequencing

doi: 10.1128/spectrum.02434-21

Figure Lengend Snippet: Schematic representation of the rearrangements of the DNA sequences of the linear plasmids from ATCC 10970, M4018, and R6-500. Rectangles, annotated transposase-related coding sequences for each plasmid sequence; Asterisk, 6.7-kb transposase fragment from the chromosome present in the M4018 plasmid; ribbons, ATCC 10970 plasmid rearrangements in M4018 and R6-500 plasmid; dotted ribbon, proposed long inverted repeat in the R6-500 plasmid; red dotted rectangle, central region of the R6-500 plasmid, consisting largely of scattered duplications; scale, size of the genomic sequences in bp.

Article Snippet: In the ATCC 10970 chromosome, this region was found to exist in two copies, first at 4680190 to 4686883 and the second at 6119904 to 6113211 bp.

Techniques: Plasmid Preparation, Sequencing, Genomic Sequencing

Journal: Microbiology Spectrum

Article Title: Reference-Grade Genome and Large Linear Plasmid of Streptomyces rimosus : Pushing the Limits of Nanopore Sequencing

doi: 10.1128/spectrum.02434-21

Figure Lengend Snippet: Schematic representation of the whole genome for strain ATCC 10970 (GenBank assembly no. GCF_006229535.1 ). First/outer ring, size of the genomic sequences in bp; second ring. Plasmid and chromosome identification. Third ring, forward/reverse CDSs; marked dots show functional RNA elements (orange, tRNA; yellow, rRNA); numbered BGCs. Forth ring, marked BGCs; orange indicates polyketide synthase-like clusters, green indicates nonribosomal peptide-like clusters, and blue indicates remaining clusters. Fifth ring, GC content (colored line) and GC average at 71.9% (black line).

Article Snippet: In the ATCC 10970 chromosome, this region was found to exist in two copies, first at 4680190 to 4686883 and the second at 6119904 to 6113211 bp.

Techniques: Genomic Sequencing, Plasmid Preparation, Functional Assay

Journal: Microbiology Spectrum

Article Title: Reference-Grade Genome and Large Linear Plasmid of Streptomyces rimosus : Pushing the Limits of Nanopore Sequencing

doi: 10.1128/spectrum.02434-21

Figure Lengend Snippet: Structures of secondary metabolites detected in the extract from S. rimosus ATCC 10970.

Article Snippet: In the ATCC 10970 chromosome, this region was found to exist in two copies, first at 4680190 to 4686883 and the second at 6119904 to 6113211 bp.

Techniques: